Two-photon fluorescence excitation spectroscopy of biological molecules

Yuri P. Meshalkin; E. E. Alfimov; D. E. Groshev; V. K. Makukha

doi:10.1117/12.242166

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10 June 1996 Two-photon fluorescence excitation spectroscopy of biological molecules

Yuri P. Meshalkin, E. E. Alfimov, D. E. Groshev, V. K. Makukha

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Proceedings Volume 2802, Laser Chemistry, Biophysics, and Biomedicine; (1996) https://doi.org/10.1117/12.242166
Event: International Conference on Coherent and Nonlinear Optics, 1995, St. Petersburg, Russian Federation

Abstract

The UV fluorescence spectra of aromatic amino-acids and some proteins at two photon excitation by second harmonic of Nd:YAG laser are received. Two-photon absorption cross sections of tryptophan, tyrosine, phenylalanine and proteins: bovine serum albumin, lysozyme, trypsin, (alpha) - chymotrypsinogen and pepsin at wavelength 532 nm were measured by means of the two-quantum standard method.

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Yuri P. Meshalkin, E. E. Alfimov, D. E. Groshev, and V. K. Makukha "Two-photon fluorescence excitation spectroscopy of biological molecules", Proc. SPIE 2802, Laser Chemistry, Biophysics, and Biomedicine, (10 June 1996); https://doi.org/10.1117/12.242166

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