Oriented bacteriorhodopsin film biomolecular devices and their photoelectric dynamics

Baoli Yao; Dalun Xu; Xun Hou

doi:10.1117/12.273484

28 May 1997 Oriented bacteriorhodopsin film biomolecular devices and their photoelectric dynamics

Baoli Yao, Dalun Xu, Xun Hou

Proceedings Volume 2869, 22nd International Congress on High-Speed Photography and Photonics; (1997) https://doi.org/10.1117/12.273484
Event: 22nd International Congress on High-Speed Photography and Photonics, 1996, Santa Fe, NM, United States

Abstract

Thin oriented bacteriorhodopsin films isolated from the Halobacterium halobium R₁M₁ strain are made on transparent conductive ITO glass and stainless sheet respectively by the use of electrophoretic sedimentation method and Langmuir-Blodgett technique. To construct photoelectric devices, another Cu or Al electrode is applied, and electrolytes or insulant films are sandwiched between the electrodes. A Q-switched double frequency Nd:YAG laser and a pulsed dye laser are used to stimulate the bacteriorhodopsin films. The photoelectric response signals are directly measured by a 300 MHz digitizing oscilloscope. Experimental curves are fitted through the use of Marquardt's algorithm least square method, and at least five exponential decay components are found in the whole process. A theory on the bacteriorhodopsin film photoelectric dynamics is proposed and the dynamic equations are mathematically derived. Comparison between the theoretical and experimental results gives the parameters of time constants and charge displacements in the bacteriorhodopsin photocycle.

Citation Download Citation

Baoli Yao, Dalun Xu, and Xun Hou "Oriented bacteriorhodopsin film biomolecular devices and their photoelectric dynamics", Proc. SPIE 2869, 22nd International Congress on High-Speed Photography and Photonics, (28 May 1997); https://doi.org/10.1117/12.273484

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