Depth-sensitive fluorescence detection of dyes in tissue phantoms

Gerd C. Beck; Willem M. Star; Hugo J. van Staveren; Lars H. P. Murrer; Angelika C. Rueck; Rudolf W. Steiner

doi:10.1117/12.260822

6 December 1996 Depth-sensitive fluorescence detection of dyes in tissue phantoms

Gerd C. Beck, Willem M. Star, Hugo J. van Staveren, Lars H. P. Murrer, Angelika C. Rueck, Rudolf W. Steiner

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260822
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

It is well known that tumors can be detected by fluorescence imaging after administration of suitable photosensitizers. The tumor's thickness, however, an important diagnostic parameter, cannot be assessed by optical methods up to now. We show that depth resolution can be achieved in the case of a fluorescent dye distributed homogeneously in a superficial region of a light scattering medium. Diffusion theory shows that the ratio of the fluorescence measured at different illumination angles is over a wide range sensitive to the thickness of the dye containing layer only, regardless of the dye concentration. For the experimental proof, a new temporally and mechanically stable tissue phantom system was developed. The experimental results on tissue phantoms confirm the predictions of diffusion theory.

Citation Download Citation

Gerd C. Beck, Willem M. Star, Hugo J. van Staveren, Lars H. P. Murrer, Angelika C. Rueck, and Rudolf W. Steiner "Depth-sensitive fluorescence detection of dyes in tissue phantoms", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260822

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available