Fluorescence microscopy studies on ALA-sensitized tissues

Gereon Huettmann; Wolfgang Achtelik; Martin Loening; Konrad Sommer; Heyke Cacile Diddens

doi:10.1117/12.260815

6 December 1996 Fluorescence microscopy studies on ALA-sensitized tissues

Gereon Huettmann, Wolfgang Achtelik, Martin Loening, Konrad Sommer, Heyke Cacile Diddens

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260815
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Fluorescence microscopy has the potential to study the spatial distribution of photosensitizers in tissue samples with cellular or subcellular resolution. A fluorescence microscope was developed to study the distribution of photosensitizer in tissue samples by acquiring fluorescence images in various spectral ranges and spatially resolved fluorescence spectra both from identical samples. Both methods provide complementary information, since the fluorescence images show the distribution of the sensitizers with a high spatial resolution whereas spatially resolved fluorescence spectra can identify the sensitizers and separate their fluorescence from background light emission by the spectral shape of the fluorescence. Protoporphyrin IX (PPIX) distribution induced by 5-aminolevulinic acid (ALA) was studied by fluorescence microscopy in basal cell carcinoma (BCC) and in cervical intraepithelial neoplasia (CIN). In an attempt to understand the varying success in treating BCC with topically applied ALA the PPIX distribution was studied in BCC samples of 10 patients. A strong fluorescence was observed in tumor cells as well as in epidermis, sebaceous glands, and hair follicles. The depth of PPIX sensitization of the BCCs ranged from 0.4 to 3 mm and the ratio of tumor versus epidermal fluorescence of uninvolved skin was near one. In the BCCs an uneven sensitization with a lower fluorescence in the center of the tumor was often observed. Samples of the cervical mucosa also showed PPIX fluorescence in the endothelial layer, the malignant tissues and the glands. No increased fluorescence of the dysplastic lesions compared to the epithelium was observed.

Citation Download Citation

Gereon Huettmann, Wolfgang Achtelik, Martin Loening, Konrad Sommer, and Heyke Cacile Diddens "Fluorescence microscopy studies on ALA-sensitized tissues", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260815

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