Light-induced translocation of Pyronine G from mitochondria to nucleoli in monkey kidney CV-1 cells

Marc Geze; M. Dellinger; M. Bazin; Rene C. Santus

doi:10.1117/12.260825

6 December 1996 Light-induced translocation of Pyronine G from mitochondria to nucleoli in monkey kidney CV-1 cells

Marc Geze, M. Dellinger, M. Bazin, Rene C. Santus

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260825
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Pyronine G (3,6-bis-N,N-dimethylaminoxanthylium chloride; PG) is a cationic dye that concentrates in mitochondria of living cells due to the high membrane potential of these organelles, similarly to rhodamine 123 and many other cationic dyes. Pyronine G also shows a preferential affinity for RNA. Upon light irradiation PG has been shown to induce cell death, but the photosensitizing properties of this molecule and the mechanism of cell death are not well understood. Microfluorometry and most particularly microspectrofluorometry are now powerful non-invasive techniques for quantitative studies of single living cells in real time which allow, for example, knowing how living cells are affected by photosensitization. To demonstrate the usefulness of image acquisition with high resolution and high sensitive camera, we present data on photosensitizer relocalization during illumination leading to functional and structural damage in the cells.

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Marc Geze, M. Dellinger, M. Bazin, and Rene C. Santus "Light-induced translocation of Pyronine G from mitochondria to nucleoli in monkey kidney CV-1 cells", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260825

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KEYWORDS

Luminescence

Kidney

Cameras

Cell death

Microscopes

Rhodamine

Data acquisition

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