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6 December 1996 Optical determination of motility forces in human spermatozoa with laser tweezers
Karsten Koenig, Lars Othar Svaasand, Yona Tadir M.D., Bruce J. Tromberg, Michael W. Berns
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Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260803
Event: BiOS Europe '96, 1996, Vienna, Austria
Abstract
Laser tweezers may act as optical force transducers. We report on the determination of intrinsic motility forces of human spermatozoa by employing an 800 nm optical trap. The cellular forces were calculated from calibrated trapping forces. The determination of trapping forces based on a hydrodynamic model for ellipsoidal specimens, the measurement of the minimum laser power required to confine a single cell in the trap, and the calculation of viscus forces during the movement of optically trapped sperm heads through a laminar fluid. A mean motility force of 44 plus or minus 24 pN was calculated for spermatozoa of healthy donors.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Karsten Koenig, Lars Othar Svaasand, Yona Tadir M.D., Bruce J. Tromberg, and Michael W. Berns "Optical determination of motility forces in human spermatozoa with laser tweezers", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260803
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KEYWORDS
Optical tweezers
Head
Calibration
Near infrared
Refractive index
Spherical lenses
Transducers
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