Polarized light microscopy technique for quantitatively mapping collagen fibril orientation in cornea

Richard H. Newton; Jacki Y. Brown; K. M. Meek

doi:10.1117/12.260805

6 December 1996 Polarized light microscopy technique for quantitatively mapping collagen fibril orientation in cornea

Richard H. Newton, Jacki Y. Brown, K. M. Meek

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260805
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Current knowledge of collagen fibril orientation in the cornea is neither quantitative, comprehensive, or consistent. Detail is lacking on how the orientation varies across the cornea and, importantly, through the thickness of the cornea. This information may be obtained from a set of corneal sections (cut normal to the corneal surface) using quantitative polarized light microscopy. The technique is based on the fact that the intensity of a corneal lamella viewed under polarized light will depend on the angle at which its component fibrils have been sectioned. Hence a measure of lamella intensity may be converted to a value for the fibril orientation relative to the plane of sectioning. In this way a contiguous map of lamella fibril orientation through the thickness of the cornea may be built up. The paper explains the technique and presents the preliminary results of the work in progress.

Citation Download Citation

Richard H. Newton, Jacki Y. Brown, and K. M. Meek "Polarized light microscopy technique for quantitatively mapping collagen fibril orientation in cornea", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260805

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