Cellulose protein films for highly specific evanescent wave immunosensors

Frank Loescher; Andreas Hartmann; J. Ueberfeld; Thomas Ruckstuhl; Daniel Bock; Thomas Jaworek; Gerhard Wegner; Stefan Seeger

doi:10.1117/12.259974

26 November 1996 Cellulose protein films for highly specific evanescent wave immunosensors

Frank Loescher, Andreas Hartmann, J. Ueberfeld, Thomas Ruckstuhl, Daniel Bock, Thomas Jaworek, Gerhard Wegner, Stefan Seeger

Author Affiliations +

Proceedings Volume 2928, Biomedical Systems and Technologies; (1996) https://doi.org/10.1117/12.259974
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

The immobilization techniques presented represent an innovative method for the highly specific determination of antigens and other proteins in a very short time and may be a useful tool for the industrial preparation of highly sensitive biosensors. Non amphiphilic alkylated cellulose films are used as matrices to reduce non-specific interactions with biomolecules. These films are transferred onto waveguides by using the Langmuir-Blodgett- technique. After photochemical stabilization the cellulose films serve as excellent matrices for the immobilization of proteins at high density. Furthermore, one-step immobilization of a mixed photopolymer antibody film with a continuously operating LB-trough is demonstrated. The activity and specificity of immobilized antibodies and streptavidin is controlled by an enzyme-linked immunosorbent assay (ELISA) and an evanescent wave immunosensor.

Citation Download Citation

Frank Loescher, Andreas Hartmann, J. Ueberfeld, Thomas Ruckstuhl, Daniel Bock, Thomas Jaworek, Gerhard Wegner, and Stefan Seeger "Cellulose protein films for highly specific evanescent wave immunosensors", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); https://doi.org/10.1117/12.259974

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available