Combined optical, electrostatic, and enzymatic handling of single DNA molecules

Carsten Hoyer; Shamci Monajembashi; Karl-Otto Greulich

doi:10.1117/12.259970

26 November 1996 Combined optical, electrostatic, and enzymatic handling of single DNA molecules

Carsten Hoyer, Shamci Monajembashi, Karl-Otto Greulich

Proceedings Volume 2928, Biomedical Systems and Technologies; (1996) https://doi.org/10.1117/12.259970
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Complete handling of single DNA molecules and their enzymatic restriction are described. For that purpose a microsphere was bound to a DNA molecule and trapped by optical tweezers. It could be moved in any direction. For stretching or rotating the molecule an electrical field was applied while the bead was fixed by the optical tweezers. In a near-equilibrium state of the resulting forces, the DNA remained stretched. Subsequently, a restriction endonuclease was activated by liberating Mg²⁺ from a caged compound. The enzymatic reaction could be directly observed in the light microscope. While the bead remained in the focus of the laser trap the other restricted part of the DNA molecule is pulled away in the electrical field. The reaction was directly monitored and recorded on videotape.

Citation Download Citation

Carsten Hoyer, Shamci Monajembashi, and Karl-Otto Greulich "Combined optical, electrostatic, and enzymatic handling of single DNA molecules", Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); https://doi.org/10.1117/12.259970

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available