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31 March 1997 Cell growth assay using the ultrasonic flexural plate-wave device
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The ability to easily monitor the rate of cell growth has many useful applications such as characterizing cell lines to optimize growth conditions and compound screening to test for cytotoxicity. As a cell growth sensor, the ultrasonic flexural plate-wave device offers a convenient assay that is non-invasive, continuous, and capable of full automation for high throughput screening. The flexural plate-wave (FPW) device is a microfabricated sensor consisting of a thin silicon nitride membrane with aluminum and zinc oxide transducers at both ends. These interdigitated transducers send and receive acoustic plate waves that propagate along the membrane. Applications of the device include mass sensing, achieved by measuring the frequency shift caused by mass loading of the membrane, and pumping of liquids and gases. We have conducted studies that established the biocompatibility of the FPW device materials with cell cultures. In addition, biological samples were exposed to the ultrasonic agitation of the device and no damaging effects were found. Experiments were performed using the FPW device to sense cell growth for mammalian suspension (non- adherent) cells. Results show a good correlation between change of cell number and frequency.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Audra H. Meng and Richard M. White "Cell growth assay using the ultrasonic flexural plate-wave device", Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997);

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