Experimental recovery of absorption, scattering, and fluorescence parameters in highly scattering media from a single frequency measurement

John S. Maier; Albert E. Cerussi; Sergio Fantini; Enrico Gratton

doi:10.1117/12.280223

18 August 1997 Experimental recovery of absorption, scattering, and fluorescence parameters in highly scattering media from a single frequency measurement

John S. Maier, Albert E. Cerussi, Sergio Fantini, Enrico Gratton

Author Affiliations +

Proceedings Volume 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II; (1997) https://doi.org/10.1117/12.280223
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

In recent years researchers have made significant progress in understanding the physics of fluorescence in highly scattering materials such as tissues in the near-infrared. We have quantitatively verified a model which describes fluorescence in ideal (homogeneous and infinite) tissue-like media. Given the quantitative accuracy of this model, one can use measurements of the fluorescence of a tissue with a homogeneous distribution of fluorophore to obtain the quantum yield, lifetime of the probe, and the absorption and scattering coefficients of the tissue at the fluorescent wavelength. We demonstrate that this can be done with a simple measurement of the photon density as a function of source- detector separation at the excitation and emission wavelengths. To verify our approach we present the lifetime, quantum yield of the fluorescent probe (rhodamine B), and the absorption and scattering coefficients of the medium at the emission peak wavelength that are obtained by a fit of the model to experimental measurements.

Citation Download Citation

John S. Maier, Albert E. Cerussi, Sergio Fantini, and Enrico Gratton "Experimental recovery of absorption, scattering, and fluorescence parameters in highly scattering media from a single frequency measurement", Proc. SPIE 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II, (18 August 1997); https://doi.org/10.1117/12.280223

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