Light scattering from cells and organelles of arbitrary shape

Andrew K. Dunn; Rebecca R. Richards-Kortum

doi:10.1117/12.280290

18 August 1997 Light scattering from cells and organelles of arbitrary shape

Andrew K. Dunn, Rebecca R. Richards-Kortum

Proceedings Volume 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II; (1997) https://doi.org/10.1117/12.280290
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Using the finite-difference time-domain technique (FDTD), the scattering patterns from cells and organelles of arbitrary shape can be computed. With this method Maxwell's curl equations are discretized in space and time and the electric and magnetic fields are computed at all points within and around the cell. The cell is constructed as a dielectric object and the far-field scattering pattern, containing both amplitude and direction information is computed. Results are presented for three-dimensional cells containing different combinations of organelles, such as nucleus, cytoplasm, and mitochondria, to assess the effect of each on the scattering pattern. The computed scattering patterns indicate that small organelles such as mitochondria play an important role in scattering from cells and variations in the refractive index of the nucleus also affect the scattering characteristics.

Citation Download Citation

Andrew K. Dunn and Rebecca R. Richards-Kortum "Light scattering from cells and organelles of arbitrary shape", Proc. SPIE 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II, (18 August 1997); https://doi.org/10.1117/12.280290

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