Analysis of particle motions by fluorescence correlation spectroscopy with traveling interference fringe excitation in biological membranes

Mineyuki Hattori; Hideaki Shimizu

doi:10.1117/12.273554

7 May 1997 Analysis of particle motions by fluorescence correlation spectroscopy with traveling interference fringe excitation in biological membranes

Mineyuki Hattori, Hideaki Shimizu

Author Affiliations +

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273554
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

A new improved method of fluorescence correlation spectroscopy, fluorescence correlation spectroscopy with traveling interference fringe excitation, for determining motional states of particles is described. In this method, the modulated fluorescence signal from particles excited by an interference fringe moving at constant velocity is detected. The autocorrelation function of the fluorescence intensity consists of terms which are characterized by the size of the excitation region and the spacing of the fringe. A lock-in detection method is adopted for extracting cosine and sine Fourier coefficients at the frequency of the traveling fringe (F_cos(t), F_sin(t)). Autocorrelation functions of F_cos(t) and F_sin(t) were found to express the motion of the fluorescent particles. The potential of this method for analyzing of the motional modes of particles in biological membranes is discussed.

Citation Download Citation

Mineyuki Hattori and Hideaki Shimizu "Analysis of particle motions by fluorescence correlation spectroscopy with traveling interference fringe excitation in biological membranes", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273554

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