Modeling single-molecule detection statistics

Joerg Enderlein; David L. Robbins; W. Patrick Ambrose; Peter M. Goodwin; Richard A. Keller

doi:10.1117/12.273570

7 May 1997 Modeling single-molecule detection statistics

Joerg Enderlein, David L. Robbins, W. Patrick Ambrose, Peter M. Goodwin, Richard A. Keller

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273570
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We present experimental results of single B-phycoerythrin molecule detection in a fluid flow at different sample introduction rates. A new mathematical approach is used for calculating the resulting burst size distributions. The calculations are based upon a complete physical model including absorption, fluorescence and photobleaching characteristics of the fluorophore; its diffusion; the sample stream hydrodynamics; the spatially dependent optical detection efficiency; and the excitation laser beam characteristics. Special attention is paid to the phenomenon of `molecular noise'--fluctuations in the number of overlapping crossings of molecules through the detection volume. The importance of this study and its connections to experimental applications are discussed.

Citation Download Citation

Joerg Enderlein, David L. Robbins, W. Patrick Ambrose, Peter M. Goodwin, and Richard A. Keller "Modeling single-molecule detection statistics", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273570

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