Detection and analysis of polymerase chain reaction products by mass spectrometry

Gregory B. Hurst; Mitchel J. Doktycz; Philip F. Britt; Arpad A. Vass; Michelle V. Buchanan

doi:10.1117/12.274345

22 May 1997 Detection and analysis of polymerase chain reaction products by mass spectrometry

Gregory B. Hurst, Mitchel J. Doktycz, Philip F. Britt, Arpad A. Vass, Michelle V. Buchanan

Proceedings Volume 2985, Ultrasensitive Biochemical Diagnostics II; (1997) https://doi.org/10.1117/12.274345
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

While molecular weight determination of proteins by matrix- assisted laser desorption/ionization (MALDI) has been quite successful, the measurement of deoxyribonucleic acid polymers in a size range useful for fine-structure genome analysis has proven more difficult, due to decreasing sensitivity and resolution at the higher molecular weights. Tailored cleanup techniques and the use of delayed extraction time-of-flight mass spectrometry are approaches that offer considerable promise for improved MALDI analysis of DNA.

Citation Download Citation

Gregory B. Hurst, Mitchel J. Doktycz, Philip F. Britt, Arpad A. Vass, and Michelle V. Buchanan "Detection and analysis of polymerase chain reaction products by mass spectrometry", Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); https://doi.org/10.1117/12.274345

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