Digital measurement of gene expression in a cDNA microarray

Edward R. Dougherty; Yidong Chen; Sinan Batman; Michael L. Bittner

doi:10.1117/12.274178

25 April 1997 Digital measurement of gene expression in a cDNA microarray

Edward R. Dougherty, Yidong Chen, Sinan Batman, Michael L. Bittner

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Proceedings Volume 3034, Medical Imaging 1997: Image Processing; (1997) https://doi.org/10.1117/12.274178
Event: Medical Imaging 1997, 1997, Newport Beach, CA, United States

Abstract

Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA micro- array. Comparison of expression levels arising from co- hybridized samples is achieved by taking ratios of average expression levels for individual genes. The present paper concerns image processing to automatically segment digitized micro-arrays and measure median gene expression levees across cDNA target sites. The main difficulty arises from determination of the target site when signal intensity is low. Segmentation must be accomplished for target sites that can possess highly unstable geometry and consist of a relatively small number of pixels. Segmentation must also be computationally efficient. The present paper proposes a nonparametric statistical method that separates target site from local background using the Mann-Whitney test.

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Edward R. Dougherty, Yidong Chen, Sinan Batman, and Michael L. Bittner "Digital measurement of gene expression in a cDNA microarray", Proc. SPIE 3034, Medical Imaging 1997: Image Processing, (25 April 1997); https://doi.org/10.1117/12.274178

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