The intracellular motility of the huge amoeboid cell, Physarum plasmodium, is defined by autowave processes, which are caused by interaction of actomyosin-based contractile apparatus in ectoplasm and intracellular endoplasmic flows. With the use of cell tensiometry and laser Doppler spectroscopy technique the autowave regimes in plasmodial strands (which look like blood-vessels) was investigated under the conditions when endogenous contractile rhythm in a part of the strand was eliminated by respiratory inhibitors. Mathematical model for ectoplasm contraction waves and shuttle endoplasm streaming in the strand is presented. Solutions of the model expanded to simulate the case of non- uniform parameter distribution satisfactorily agree with the experimentally observed effects of the respiratory inhibitors.

A coherent optics technique of obtaining heart beats time series is presented. The method uses a dependence of properties of speckle-structures formed by focused coherent light field diffraction from a rough surface on the surface statistics and movement parameters. Computer simulation was applied to analyze different components of skin surface vibration and their influence on speckles dynamics was investigated.

On the base of computer simulation the immune kinetic processes for in vitro analysis was carried out for two cases: (1) antigen (Ag) was polyvalent, antibody (Ab)- monovalent; and (2) both Ag and Ab were bivalent. The problem was solved by calculation of the differential equations system minding limitations naturally. The immune reaction was treated as a combination of two competing processes: the antigen-antibody aggregation and synchronous disaggregation of immune complexes. Such a combination is to be described by a system of nonlinear differential equations but for simplicity the equations were linearized. The solution of the differential equation system was carried out minding some limitations naturally. The kinetic curves of immune reaction were obtained for different initial Ag, Ab concentrations and two experimental procedures. Partially, the concentrational curves obtained occurred for some cases analogous to the well known experimental results -- 'zone of equivalence.' The results of in vitro immune reaction simulation were used for computer analysis of one of the widely used methods of immune biomedical diagnosis -- light beam scattering (nephelometry). The light beam scattering by the 'Ag-Ab-complex' mixture during immune reaction was treated as a single light scattering on the spheres of different diameters less than the wavelength. So the scattering medium was the polydisperse system, when the scattering centers concentration and sizes were changing in time. The expression for intensity of scattered light induced by the immune reaction process for different experimental conditions was derived.

Changes of light scattering matrix elements during the aggregation process are investigated. It is shown that during the aggregation the depolarizing ability of protein solution increases for the particles smaller than wavelength and it may have a minimum for the larger ones. Light depolarization is estimated as a function of the measurement duration and of the width of particles distribution by size.

We have non-invasively measured in vivo blood flow changes using photon correlation spectroscopy with a simple correlation diffusion model to study and quantify blood flow in the human arm during cuff ischemia. The method utilizes the Doppler broadening of light that arises in a multiply scattering dynamic media, and is also responsive to changes in absorption and scattering coefficients. Our measurements clearly show blood flow changes with cuff pressures, including the hyperemic overshoot after cuff release, that qualitatively agree with the physiological behavior of the cardiovascular system. In this paper, we present our method, results and discuss the clinical relevance of our findings.

Results of Monte-Carlo simulations of Doppler shift are presented for model of random medium that containing moving particles. The single-layered and two-layered configurations of the medium are considered. Doppler shift of the frequency of incident laser light is investigated as a function of such parameters as absorption coefficient, scattering coefficient, and thickness of the medium.

The possibility of application of speckle interferometry for diagnostics in dentistry has been analyzed. The problem of standardization of the measuremental procedure has been studied. An algorithm of processing of Doppler signal in the diagnostics of blood microcirculation in mucous membrane have been suggested.

The issues about the behavior of variant test particles (TP) in 2D potential field with complex relief are studied. The cases with time-dependent parameters of potential field are considered. In particular the mathematical models that are discussed in this paper can describe either the process of penetration of TP (ligand) in active site of the enzyme or proton's transition from one potential pit to another.

The results of the experimental investigation of the erythrocyte functional states evaluated by mechanical parameters of the erythrocyte membranes and biochemical indices of the redox reactions are reported. A spatial image is obtained for different states of the membranes. It has the form of a multilevel structure consisting of discrete self-similar domains, the transitions between which occur through membrane restructuring of two types. The specific features of molecule interactions between membrane components and the character of their changes dependent of the localization of the membrane states domain in the multilevel structure are discussed.

An approach to description of scattered light intensity fluctuations as the generalized Brownian process is discussed. These fluctuations can be caused by the regular movement of the scattering objects with respect to coherent probe beam. Exponential factor of the structure function of the scattered light intensity fluctuations is proposed as the generalized parameter for characterization of the scattering properties of biotissues. Manifestations of the effect of chaotization of the scattered light intensity fluctuations associated with the decreasing of exponential factor (and, correspondingly, with the increasing of Hausdorff dimension) are experimentally studied for the case of model scatterers (rough glass plates) with fractal properties of surface relief and non-single scattering tissues such as human sclera. In the last case transition from multiple scattering to single scattering mode stimulated by special chemical agent (Trazograph solution) is experimentally studied.

Effects of appearance of speckle contrast maxima in the far diffraction zone have been studied for the case of single scattering structures with fractal distributions of boundary field phase. Band-limited Weierstrass function has been chosen as the scattering structure model. One of the possible applications of the presented below analysis is the study of the biotissues scattering structures or scattering surfaces with complex topography. Obtained results seem very prospective for the development of the coherent diagnostic technique on the basis of speckle intensity statistical analysis.

Analytical simulation model for description of speckle intensity fluctuations which is based on the one-dimensional band-limited Weierstrass function, is discussed. Certain 'non-linearity' as the dependence of Weierstrass function parameter on its argument is introduced to simulate large- scale inhomogeneities. Reconstruction of scattering structures images on the basis of speckle correlometric technique is studied using the developed model.

Experiments on studying of the dynamics of luminescence intensity under continuous 360 plus or minus 20 nm irradiation were conducted on normal human epidermis sampled using surface epidermal stripping technique and treated with 8-MOP in vitro. Alterations in luminescence spectra after one hour continuous UV irradiation differ in pure and psoralen treated epidermis. Kinetic curves (luminescence intensity at different wavelengths versus time of irradiation) for 8-MOP treated epidermis have the period of luminescence 'glowing up' which covers first 300 - 400 sec of irradiation time then much more gently stopping bleaching region which goes in intensity lower than initial (before irradiation) level. In the frame of the kinetic scheme for luminescent photoproduct, comprising two consecutive first order reactions, values of rate constants of luminescence 'growing up' and diminution were calculated.

A new measuring technique of statistical parameters of transparent or reflecting random phase objects including biological tissue is discussed. The technique is based on the usage of the probing spatially modulated laser beam (beam with regular interference pattern), focused on a moving object, and on measurement of contrast of average intensity fringes, formed in scattered dynamic speckle- modulated field. The behavior of the speckle field interference induced by the focused spatially modulated laser beam diffraction on the random phase objects has been studied. The dependence of dynamic field average intensity fringes contrast on the statistical properties of moving object has been established both experimentally and theoretically.

The theoretical model of a human eye lens is presented. This model accounts effects of the spatial interaction of scatterers arising due to existence of closely-packed system of particles and the complexity of refractive index of their material. The calculations of transmission and scattering spectra for a monodispersive and polydispersive systems of scatterers were done. The influence of absorption on transmission spectra formation was analyzed.

We report on the experimental results concerning the regimes of transition to the spatial-temporal chaos in an optical system with the delayed feedback based on the CCD camera and TV display. The analogy between the observed optical instabilities and the complex oscillations in the human pupil light reflex are discussed.

In the work the mathematical model is presented which describes the processes in the epidermis sensitized by the furocoumarins under UVA-radiation. The model describes the processes at three levels: molecular (the photochemical transformations of the psoralen molecules and their reactions with the DNA-molecules), cellular (proliferation, differentiation and repairing of injuries), and tissue (humoral regulation, space-time cell dynamics in the epidermis). The results of the numerical simulations of both the latent period and psoriasis manifestation are given. The therapeutical effect of the UV-radiation is considered in the framework of the model.

We studied autofluorescence of the layers of normal human epidermis in the course of the development of less invasive technique for assessment of tissue state. Surface epidermal stripping technique was applied to spectrofluorimetric study of epidermis. Three bands (280 plus or minus 20 nm, 360 plus or minus 20 nm, 435 plus or minus 20 nm) were used for excitation of sample fluorescence. Some well reproducible autofluorescence bands were observed over all samples. Data averaged over the group of volunteers are indicative of the decrease of intrinsic epidermis fluorophores content with depth. Alterations in fluorescence of samples of different epidermal layers under storage were studied as well as the dynamics of autofluorescence degradation in just taken strippings under continuous UV irradiation.

In experiments on isolated amphibian hearts changes in photoreactivity of pacemaker cells under the influence of He-Ne laser radiation in different phases of the heart cycle were studied. The specificity of heart photoreaction, peculiarities of relaxation period after laser light action and laser modification of hypodynamic depression development were revealed. Adaptation of pacemaker cells to the He-Ne laser exposure was observed.

Experimental and clinical studies carried out with the help of optical, biochemical, potentiometric and EPR-methods showed that blood reaction to light irradiation is similar to that of nonequilibrium medium characterized by oscillating and multistationary regimes that makes it impossible to a priori predict the only possible reaction. The mechanism of biological effect of low-intensity light is realized through deviations in oxidation-reduction blood system balance due to selective activation of these or those key components which include, in particular, compounds containing Fe and Cu ions. Value and sign of these reactions are determined by the state of this system at each time moment and are nontrivially associated with the light of different wavelength ranges. A possibility to predict the reaction of the patient to phototherapy based on the results of preliminary irradiation of his blood in vitro is shown. The technique and laboratory criteria making it possible to predict directivity and efficiency of the patient's reaction to future-to-be irradiation (irrespective of the manner of irradiation) and to choose the required wavelength range providing for the optimal therapeutic effect at this particular time moment is worked out.

Analysis of reflectance and autofluorescence spectra of in vivo human skin with eruthema was done. Monte Carlo simulation was used to model the tissue R and AF measurements. As a result the information about qualitative and quantitative changes of the main human skin chromophores, such as hemoglobin and melanin are, distribution within the skin tissue during the process of UV irradiation of the skin was obtained. The metabolic nature of investigated nonlinear process of UV radiation influence on the skin tissue can call interest as a problem of nonlinear dynamics.

Experiments on white rats with wounds have shown stage changes in glycolytic and redox reactions occurring in a wound. Wound healing after repeated irradiation of wounds with infrared laser was marked by the emergence of some metabolic effects. The latter ones included glycolysis activation after 3 sessions of irradiation followed by more rapid normalization of glycolytic and redox processes.