Fluorescence imaging and chlorophyll fluorescence to evaluate the role of EDU in UV-B protection in cucumber

Ravinder K. Sandhu; Moon S. Kim; Donald T. Krizek; Elizabeth M. Middleton

doi:10.1117/12.277617

2 July 1997 Fluorescence imaging and chlorophyll fluorescence to evaluate the role of EDU in UV-B protection in cucumber

Ravinder K. Sandhu, Moon S. Kim, Donald T. Krizek, Elizabeth M. Middleton

Author Affiliations +

Proceedings Volume 3059, Advances in Laser Remote Sensing for Terrestrial and Oceanographic Applications; (1997) https://doi.org/10.1117/12.277617
Event: AeroSense '97, 1997, Orlando, FL, United States

Abstract

A fluorescence imaging system and chlorophyll fluorescence emissions were used to evaluate whether EDU, N-[2-(2-oxo-1- imidazolidinyl) ethyl]-N'-phenylurea, provided protection against ultraviolet-B (UV-B) irradiation (290 - 320 nm) in cucumber (Cucumis sativus L.) leaves. Plants were grown in growth chambers illuminated for 14 h per day with 400 W high pressure sodium and metal halide lamps. Photosynthetically active radiation (PAR) for 1 hr at the beginning and end of each cycle was provided at 270 micrometers ol m^-2 s^-1 PAR; during the other 12 hr of the photoperiod, the plants received 840 micrometers ol m^-2 s^-1 PAR. Beginning on the twelfth day, the plants were exposed to UV-B radiation (0.2 & 18.0 kJ m^-2d^-1) for 2 days at 8 h per day centered in the photoperiod. Rapidly acquired (less than 1 s), high spatial resolution (less than 1 mm²) images were obtained for whole adaxial leaf surfaces using a fluorescence imaging system. The steady-state fluorescence images were acquired in four spectral regions: blue (F450 nm), green (F550 nm), red (F680 nm), and far-red (F740 nm). Fluorescence emission spectra for leaf pigments extracted in dimethyl sulfoxide (DMSO) were obtained by excitation at 280 and 380 nm (280EX 300 - 530 nm; 380EX 400 - 800 nm). Both UV-B and EDU induced stress responses in cucumber leaves that altered the fluorescence emissions obtained from extracts. In the fluorescence images only UV-B induced stress responses were observed but this damage was detected before it was visually apparent. There was no evidence that EDU afforded protection against UV-B irradiation. Use of fluorescence imaging may provide an early stress detection capability for helping to assess damage to the photosynthetic apparatus of plants.

Citation Download Citation

Ravinder K. Sandhu, Moon S. Kim, Donald T. Krizek, and Elizabeth M. Middleton "Fluorescence imaging and chlorophyll fluorescence to evaluate the role of EDU in UV-B protection in cucumber", Proc. SPIE 3059, Advances in Laser Remote Sensing for Terrestrial and Oceanographic Applications, (2 July 1997); https://doi.org/10.1117/12.277617

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