Optical biosensing using surface plasmon resonance spectroscopy

Jiri Homola; Peter Pfeifer; Eduard Brynda; Jiri Skvor; Milan Houska; Guenter Schwotzer; Ines Latka; Reinhardt Willsch

doi:10.1117/12.276167

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30 May 1997 Optical biosensing using surface plasmon resonance spectroscopy

Jiri Homola, Peter Pfeifer, Eduard Brynda, Jiri Skvor, Milan Houska, Guenter Schwotzer, Ines Latka, Reinhardt Willsch

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Proceedings Volume 3105, Chemical, Biochemical and Environmental Fiber Sensors IX; (1997) https://doi.org/10.1117/12.276167
Event: Environmental Sensing III, 1997, Munich, Germany

Abstract

The exploitation of surface plasmon resonance (SPR) sensing principle for the study of formation of multilayers of human immunoglobulin (hlgG) and monoclonal antibody against human choriogonadotropin (a-hCG) as well as for monitoring immunoreaction between the immobilized a-hCG and human choriogonadotropin (hCG) is reported. It is demonstrated that even a very small attachment of proteins (due to the adsorption or the specific interaction) may be detected by the presented SPR sensor. An immobilization technique is described which allows to form stable multilayers of antibodies on the gold surface.

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Jiri Homola, Peter Pfeifer, Eduard Brynda, Jiri Skvor, Milan Houska, Guenter Schwotzer, Ines Latka, and Reinhardt Willsch "Optical biosensing using surface plasmon resonance spectroscopy", Proc. SPIE 3105, Chemical, Biochemical and Environmental Fiber Sensors IX, (30 May 1997); https://doi.org/10.1117/12.276167

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