Color image analysis of contaminants and bacteria transport in porous media

Mehdi Rashidi; Jamshid Dehmeshki; Mohammad Farhang Daemi; Larry Cole; Eric Dickenson

doi:10.1117/12.284200

24 October 1997 Color image analysis of contaminants and bacteria transport in porous media

Mehdi Rashidi, Jamshid Dehmeshki, Mohammad Farhang Daemi, Larry Cole, Eric Dickenson

Proceedings Volume 3159, Algorithms, Devices, and Systems for Optical Information Processing; (1997) https://doi.org/10.1117/12.284200
Event: Optical Science, Engineering and Instrumentation '97, 1997, San Diego, CA, United States

Abstract

Transport of contaminants and bacteria in aqueous heterogeneous saturated porous systems have been studied experimentally using a novel fluorescent microscopic imaging technique. The approach involves color visualization and quantification of bacterium and contaminant distributions within a transparent porous column. By introducing stained bacteria and an organic dye as a contaminant into the column and illuminating the porous regions with a planar sheet of laser beam, contaminant and bacterial transport processes through the porous medium can be observed and measured microscopically. A computer controlled color CCD camera is used to record the fluorescent images as a function of time. These images are recorded by a frame accurate high resolution VCR and are then analyzed using a color image analysis code written in our laboratories. The color images are digitized this way and simultaneous concentration and velocity distributions of both contaminant and bacterium are evaluated as a function of time and pore characteristics. The approach provides a unique dynamic probe to observe these transport processes microscopically. These results are extremely valuable in in-situ bioremediation problems since microscopic particle-contaminant- bacterium interactions are the key to understanding and optimization of these processes.

Citation Download Citation

Mehdi Rashidi, Jamshid Dehmeshki, Mohammad Farhang Daemi, Larry Cole, and Eric Dickenson "Color image analysis of contaminants and bacteria transport in porous media", Proc. SPIE 3159, Algorithms, Devices, and Systems for Optical Information Processing, (24 October 1997); https://doi.org/10.1117/12.284200

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