Enhancing growth of cultured human skin cells using low-energy CO2 laser

Nili Grossman; Haim Reuveni; Sima Halevy; Rachel Lubart

doi:10.1117/12.298002

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22 December 1997 Enhancing growth of cultured human skin cells using low-energy CO₂ laser

Nili Grossman, Haim Reuveni, Sima Halevy, Rachel Lubart

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Proceedings Volume 3198, Effects of Low-Power Light on Biological Systems; (1997) https://doi.org/10.1117/12.298002
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

In view of the versatility and usage of the CO₂ laser as a too. in surgery and dermatology, we have studied its effect on enhancing proliferation of cultured skin cells using an attenuated CO₂ laser. Exposure of cultured keratinocytes or fibroblasts to continuous wave or pulse mode irradiation enhanced thymidine incorporation by 1.4 to 1.7 folds, and cell number by 1.25 to 1.4 folds, measured 24 and 48 hours later, depending on the fluency applied. As expected, these effects were not suppressed by added antioxidants, indicating that the mechanism involved in this newly observed effect, differ from photosensitization by low energy visible and near IR lasers.

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Nili Grossman, Haim Reuveni, Sima Halevy, and Rachel Lubart "Enhancing growth of cultured human skin cells using low-energy CO₂ laser", Proc. SPIE 3198, Effects of Low-Power Light on Biological Systems, (22 December 1997); https://doi.org/10.1117/12.298002

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