Low-cost endothelium cell counter for slit lamp

Liliane Ventura; Sidney Julio Faria de Sousa; Paulo E. M. Ribeiro Jr.; Cesar A. Cardosa Caetano

doi:10.1117/12.309440

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1 June 1998 Low-cost endothelium cell counter for slit lamp

Liliane Ventura, Sidney Julio Faria de Sousa, Paulo E. M. Ribeiro Jr., Cesar A. Cardosa Caetano

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Proceedings Volume 3246, Ophthalmic Technologies VIII; (1998) https://doi.org/10.1117/12.309440
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

One of the optical ways to evaluate the donated cornea in order to provide a diagnostic regarding its indication for transplant is to count the number of the living endothelial cells (over 2000 cells/mm²), which are responsible for maintaining the corneal transparency. Specular Microscopes are equipments which are exclusively dedicated for this kind of evaluation. However they are of high cost and most of the Eye Banks are not provided by them. Hence, the usual evaluation is done in a Slit Lamp (SL) -- 40X magnification -- and just the aspect of the cells are subjectively observed. In order to overcome the limitations of subjective assessment and high cost, we have developed a system attached to the SL (optical system with 250X magnification image captured by a CCD detector which displays the image of the cells on a PC monitor and a dedicated software) which is able to count the endothelial cells providing a lower cost objective diagnostic of the donated cornea.

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Liliane Ventura, Sidney Julio Faria de Sousa, Paulo E. M. Ribeiro Jr., and Cesar A. Cardosa Caetano "Low-cost endothelium cell counter for slit lamp", Proc. SPIE 3246, Ophthalmic Technologies VIII, (1 June 1998); https://doi.org/10.1117/12.309440

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