Elastic light scattering on reagent mixture in the immune reaction process in vitro

Valeri A. Doubrovski; K. N. Dvoretski; Vyacheslav F. Kirichuk; I. V. Scherbakova

doi:10.1117/12.306052

24 April 1998 Elastic light scattering on reagent mixture in the immune reaction process in vitro

Valeri A. Doubrovski, K. N. Dvoretski, Vyacheslav F. Kirichuk, I. V. Scherbakova

Proceedings Volume 3251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications II; (1998) https://doi.org/10.1117/12.306052
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The paper is devoted to computer simulation of elastic light scattering on reagent mixture during the immune reaction process in vitro. The immune reaction was analyzed in the limits of the 3-stages process of antigen-antibody complexes aggregation. Antibodies were treated as bivalent biomolecules, antigens were performed by 2, 3-valent biomolecule or polyvalent cells. Two variants of the problem solution of light scattering on the biological objects chosen were analyzed-Rayleigh-Gans-theory, the approach, and Mie theory, the preference was given to the first one. The optimum of the immune reaction output was revealed at quite a definite ratio of initial reagents concentrations. This optimum is analog of experimentally a well known phenomenon -- the 'zone of equivalence.' It's shown that the form of the 'zone of equivalence' both depends upon the kind of experimental procedure and the ratio of the antigen, antibody sizes. This result is preferably to take into consideration while one tries to optimize the medical diagnostic procedure based on the immune approach. It was revealed that the scattered light power correlates with the immunochemical kinetics not adequately sometimes. That fact may be useful to interpret the experimental investigations and at the same time it may give a principal possibility to use nephelometry for the aims of immune metrology.

Citation Download Citation

Valeri A. Doubrovski, K. N. Dvoretski, Vyacheslav F. Kirichuk, and I. V. Scherbakova "Elastic light scattering on reagent mixture in the immune reaction process in vitro", Proc. SPIE 3251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications II, (24 April 1998); https://doi.org/10.1117/12.306052

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