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1 May 1998 Resolution degradation due to brain tissue scattering
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Proceedings Volume 3253, Biomedical Sensing and Imaging Technologies; (1998)
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States
We have investigated the imaging of structural details at various depths within rat brain tissue to better understand the loss of resolution and contrast due in large part to light scattering. A target was imaged through various thicknesses of tissue; the normalized slope of transition between light and dark regions of the images was used as an effective resolution index. The ERI improves dramatically as the wavelength is increased: 0.03 at 600nm, 0.3 at 850nm for a 270 micrometers -thick hippocampus slice. A comparable change was noted for a 270 micrometers -thick cortex slice. For a fixed wavelength, ERI decreases for thicker hippocampus slices, form 0.67 at 220 micrometers to 0.31 at 250 micrometers and to 0.24 at 300 micrometers . Image contrast improves with longer wavelength: from 0.9 at 600nm to 9.5 at 850 nm; from 0.9 at 600nm to 4.6 at 850nm. Despite regional differences in the transparent of brain regions, images were degraded less by scattering at longer wavelengths, arguing strongly for the use of near-IR wavelengths for microscopic imaging either with transmitted light or fluorescent emission for exploring deeper biological structures.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Chun-Ming Albert Wang, J. Harold Wayland, and Scott E. Fraser "Resolution degradation due to brain tissue scattering", Proc. SPIE 3253, Biomedical Sensing and Imaging Technologies, (1 May 1998);

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