Photodynamic therapy affects the expression of IL-6 and IL-10 in vivo

Sandra O. Gollnick; David A. Musser; Barbara W. Henderson

doi:10.1117/12.308168

13 May 1998 Photodynamic therapy affects the expression of IL-6 and IL-10 in vivo

Sandra O. Gollnick, David A. Musser, Barbara W. Henderson

Proceedings Volume 3254, Laser-Tissue Interaction IX; (1998) https://doi.org/10.1117/12.308168
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Photodynamic therapy (PDT), which can effectively destroy malignant tissue, also induces a complex immune response which potentiates anti-tumor immunity, but also inhibits skin contact hypersensitivity (CHS) and prolongs skin graft survival. The underlying mechanisms responsible for these effects are poorly understood, but are likely to involve meditation by cytokines. We demonstrate in a BALB/c mouse model that PDT delivered to normal and tumor tissue in vivo causes marked changes in the expression of cytokines interleukin (IL)-6 and IL-10. IL-6 mRNA and protein are rapidly and strongly enhanced in the PDT treated EMT6 tumor. Previous studies have shown that intratumoral injection of IL- 6 or transduction of the IL-6 gene into tumor cells can enhance tumor immunogenicity and inhibit tumor growth in experimental murine tumor systems. Thus, PDT may enhance local anti-tumor immunity by up-regulating IL-6. PDT also results in an increase in IL-10 mRNA and protein in the skin. The same PDT regime which enhances IL-10 production in the skin has been shown to strongly inhibit the CHS response. The kinetics of IL-10 expression coincide with the known kinetics of PDT induced CHS suppression and we propose that the enhanced IL-10 expression plays a role in the observed suppression of cell mediated responses seen following PDT.

Citation Download Citation

Sandra O. Gollnick, David A. Musser, and Barbara W. Henderson "Photodynamic therapy affects the expression of IL-6 and IL-10 in vivo", Proc. SPIE 3254, Laser-Tissue Interaction IX, (13 May 1998); https://doi.org/10.1117/12.308168

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