Light scattering from dimers: latex-latex and gold-latex

Kirk A. Fuller; Reinaldo J. Gonzalez; Manish S. Kochar

doi:10.1117/12.307062

1 May 1998 Light scattering from dimers: latex-latex and gold-latex

Kirk A. Fuller, Reinaldo J. Gonzalez, Manish S. Kochar

Proceedings Volume 3256, Advances in Optical Biophysics; (1998) https://doi.org/10.1117/12.307062
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

This paper presents theoretical modeling and experimental results of light scattering studies on latex (polystyrene) particle dimers and colloidal gold particles coupled to individual latex particles. The latex particle size range is 1 - 10 micrometer (in diameter) and that of the gold particles is 80 - 100 nm. In general, the latex particles are spherical, while the colloidal gold particles have varied shapes. The theoretical model is based on exact solution of the problem of light scattering by interacting spheres. Calculations based on interacting and noninteracting particles will be compared to experimental results. The experimental results were obtained with high resolution light scattering measurements of particle coupling in a flow particle analyzer. This analyzer, Copalis^TM (Coupled Particle Light Scattering), exploits particle coupling induced by antigen-antibody, receptor- hormone, or nucleic acid interactions in medical diagnostic assays. Although latex particle coupling produces a range of aggregate sips, only dimer light scattering results are relevant. Coupling of gold to latex particles in the Copalis^TM diagnostic system produces from one to many gold particles per latex particle. Coherent addition of fields scattered by many gold-latex pairs has been used to simulate the gold-based assay. The comparison of theoretical simulations and experimental results for the coupling will be discussed. The results of this study are being used to refine the particle coupling results in the Copalis^TM diagnostic system.

Citation Download Citation

Kirk A. Fuller, Reinaldo J. Gonzalez, and Manish S. Kochar "Light scattering from dimers: latex-latex and gold-latex", Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); https://doi.org/10.1117/12.307062

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available