Microfabricated electric impedance chamber for the electrical characterization of single cells

H. Edward Ayliffe; Richard D. Rabbitt; A. Bruno Frazier

doi:10.1117/12.304366

26 March 1998 Microfabricated electric impedance chamber for the electrical characterization of single cells

H. Edward Ayliffe, Richard D. Rabbitt, A. Bruno Frazier

Proceedings Volume 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications; (1998) https://doi.org/10.1117/12.304366
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Micromachining technologies were applied to fabricate metal- electrode-instrumented microchannels with cross-sectional dimensions similar in size to blood cells. The instruments enable electric impedance measurement of femptoliter quantities of materials and solutions. The completed micro- electric impedance devices were characterized with varying concentrations of phosphate buffered saline solutions, DI water, and air in the recording zone. With the microdevices on the stage of an inverted light microscope, individual living cells were positioned tightly between metal electrodes using mechanical suction. Impedance spectra form 100 Hz to 2 MHz measured in isolated toadfish red blood cells (RBCs) and human neutrophils were distinct and demonstrated the ability to permeate the cell membrane at high frequency. The cell/shunt path cut-off frequency were approximately 400 kHz at -3dB indicating that non- invasive electric impedance characterization of the cytoplasm may be feasible for his configuration. In addition, the area specific membrane capacitance was estimated for both cell types by fitting the data to a simple RC circuit model.

Citation Download Citation

H. Edward Ayliffe, Richard D. Rabbitt, and A. Bruno Frazier "Microfabricated electric impedance chamber for the electrical characterization of single cells", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); https://doi.org/10.1117/12.304366

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