Automated spectral imaging for clinical diagnostics

John Breneman; David M. Heffelfinger; Ken Pettipiece; Chris Tsai; Peter Eden; Richard A. Greene; Karen J. Sorensen; Will Stubblebine; Frank Witney

doi:10.1117/12.307320

10 April 1998 Automated spectral imaging for clinical diagnostics

John Breneman, David M. Heffelfinger, Ken Pettipiece, Chris Tsai, Peter Eden, Richard A. Greene, Karen J. Sorensen, Will Stubblebine, Frank Witney

Author Affiliations +

Proceedings Volume 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery; (1998) https://doi.org/10.1117/12.307320
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Bio-Rad Laboratories supplies imaging equipment for many applications in the life sciences. As part of our effort to offer more flexibility to the investigator, we are developing a microscope-based imaging spectrometer for the automated detection and analysis of either conventionally or fluorescently labeled samples. Immediate applications will include the use of fluorescence in situ hybridization (FISH) technology. The field of cytogenetics has benefited greatly from the increased sensitivity of FISH producing simplified analysis of complex chromosomal rearrangements. FISH methods for identification lends itself to automation more easily than the current cytogenetics industry standard of G- banding, however, the methods are complementary. Several technologies have been demonstrated successfully for analyzing the signals from labeled samples, including filter exchanging and interferometry. The detection system lends itself to other fluorescent applications including the display of labeled tissue sections, DNA chips, capillary electrophoresis or any other system using color as an event marker. Enhanced displays of conventionally stained specimens will also be possible.

Citation Download Citation

John Breneman, David M. Heffelfinger, Ken Pettipiece, Chris Tsai, Peter Eden, Richard A. Greene, Karen J. Sorensen, Will Stubblebine, and Frank Witney "Automated spectral imaging for clinical diagnostics", Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); https://doi.org/10.1117/12.307320

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KEYWORDS

Optical filters

Spectroscopy

Luminescence

Image filtering

Microscopes

Charge-coupled devices

Imaging spectroscopy

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