Comparison between time-correlated single-photon counting and fluorescence correlation spectroscopy in single-molecule identification

Joerg Enderlein; Malte Koellner

doi:10.1117/12.307340

10 April 1998 Comparison between time-correlated single-photon counting and fluorescence correlation spectroscopy in single-molecule identification

Joerg Enderlein, Malte Koellner

Author Affiliations +

Proceedings Volume 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery; (1998) https://doi.org/10.1117/12.307340
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Currently, two methods of detection and identification of single molecules are widely used: fluorescence correlation spectroscopy (FCS) and time-correlated single photon counting (TCSPC). We present a thorough theoretical analysis of the error rates for identifying single molecules according to their diffusion coefficients (using FCS), and to their fluorescence lifetimes (using TCSPC). In most cases, the error rate using TCSPC is much lower. TCSPC is thus proven to be more versatile for analyzing single molecule events. The study is significant for a broad range of ultrasensitive fluorescence detection applications.

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Joerg Enderlein and Malte Koellner "Comparison between time-correlated single-photon counting and fluorescence correlation spectroscopy in single-molecule identification", Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); https://doi.org/10.1117/12.307340

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