Quantitative imaging of assays in miniaturized plate formats

Gang Sun; Peter Ramm

doi:10.1117/12.307331

10 April 1998 Quantitative imaging of assays in miniaturized plate formats

Gang Sun, Peter Ramm

Proceedings Volume 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery; (1998) https://doi.org/10.1117/12.307331
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Miniaturized sample formats offer advantages in cost and throughput to screening laboratories. Imaging systems may be valuable tolls for assay miniaturization. We describe fluorescence performance of our Tundra^TM assay imaging system, which incorporates a thinned, back illuminated CCD area detector, a telecentric lens with internal excitation optics, and AssayVision^TM software. Using a flat field correction, Tundra imaged a difficult (faint) gel with performance similar to that of a high performance scanning laser. The accuracy of Tundra's dedicated well plate calibration functions was also evaluated in 384 (40 (mu) l sample volume) and 1536 (5 (mu) l) well plates, and across a realistic range of fluor concentrations. With both plate formats, and across the full range of fluor concentrations, Tundra shows coefficients of variation which are similar to those of a scanning laser, and to those reported for PMT devices. Tundra also exhibits highly linear response to fluor concentration.

Citation Download Citation

Gang Sun and Peter Ramm "Quantitative imaging of assays in miniaturized plate formats", Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); https://doi.org/10.1117/12.307331

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available