Optically sectioned images in wide-field fluorescence microscopy

Tony Wilson; Mark A. A. Neil; Rimas Juskaitis

doi:10.1117/12.310538

9 June 1998 Optically sectioned images in wide-field fluorescence microscopy

Tony Wilson, Mark A. A. Neil, Rimas Juskaitis

Proceedings Volume 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V; (1998) https://doi.org/10.1117/12.310538
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.

Citation Download Citation

Tony Wilson, Mark A. A. Neil, and Rimas Juskaitis "Optically sectioned images in wide-field fluorescence microscopy", Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); https://doi.org/10.1117/12.310538

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