mTHPC-mediated photodynamic detection for fluorescence-guided resection of brain tumors

Herwig Kostron; Andreas Zimmermann; Alois Obwegeser

doi:10.1117/12.309486

5 June 1998 mTHPC-mediated photodynamic detection for fluorescence-guided resection of brain tumors

Herwig Kostron, Andreas Zimmermann, Alois Obwegeser

Proceedings Volume 3262, Surgical-Assist Systems; (1998) https://doi.org/10.1117/12.309486
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

A most radical resection is of great importance in the treatment of brain tumors, however they can hardly be differentiated from normal brain parenchyma by the naked eye of the neurosurgeon. Photosensitizers are highly selective taken up into malignant tissues, therefore the fluorescence properties of photosensitizers could be utilized for optical differentiation of normal and malignant tissue. Ten patients presenting with brain malignancies were sensitized for photodynamic diagnosis (PDD) and photodynamic treatment (PDT) with 0.15 mg/kg b.w. m-THPC. On day 4 intraoperative PDD and fluorescence guided tumor resection (FGR) was performed, followed by intraoperative PDT. The fluorescence was induced by a xenon lamp at an excitation wavelength ranging from 370 to 440 nm. A sensitive CCD camera was employed for imaging, equipped with a long pass filter to shut off the excitation wavelength and to improve the signal to noise ratio. The pictures are converted digitally by a standard frame grabber and processed in real time and calculated for the tissue auto fluorescence in the emission band of m-THPC at 652 nm. Out of 10 0bservations, two were false negative and 2 were false positive. Our preliminary results indicate that fluorescence guided surgery is feasible and proved to be of significant help in delineating tumor margins and in resection of residual tumor that could not be detected by the surgeon, however the sensitivity and specificity needs to be further improved.

Citation Download Citation

Herwig Kostron, Andreas Zimmermann, and Alois Obwegeser "mTHPC-mediated photodynamic detection for fluorescence-guided resection of brain tumors", Proc. SPIE 3262, Surgical-Assist Systems, (5 June 1998); https://doi.org/10.1117/12.309486

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