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23 February 1999 Development of oxygen and pH optical sensors using phase modulation technique
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Proceedings Volume 3540, Chemical, Biochemical, and Environmental Fiber Sensors X; (1999) https://doi.org/10.1117/12.339803
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States
Abstract
In this paper we report the use of phase sensitive fluorometry to obtain preliminary results from micrometer- sized optical fiber oxygen sensors and a pH sensitive dye. Micrometer-sized (100 micrometers and 10 - 20 micrometers ) oxygen sensors were prepared on the distal tip of an optical fiber using a dip-coating procedure to immobilize Tris(4,7- Diphenyl-1,10-Phenanthroline)Ru(II) Chloride (Ru(dpp)) within a sol-gel matrix. These optodes exhibit excellent performance towards both gaseous and dissolved oxygen. The sensor's intensity based excitation and emission spectra, as well as phase fluorometric measurements, were collected utilizing a back-detection set-up. These micro-optodes have many desirable features for minimally invasive bio-medical applications arising from the potential of miniaturization. Carboxynaphthofluorescein solutions are shown to exhibit pH- dependent fluorescence decay times and analysis of the phase and the modulation values indicate a strong pH dependence in the physiological range, thus identifying this water-soluble dye as an attractive candidate for optical pH measurements in biomedical samples.
© (1999) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Zoe Chen-Esterlit, Jonathan W. Aylott, and Raoul Kopelman "Development of oxygen and pH optical sensors using phase modulation technique", Proc. SPIE 3540, Chemical, Biochemical, and Environmental Fiber Sensors X, (23 February 1999); https://doi.org/10.1117/12.339803
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