Changes in autofluorescence emission intensities of human colonic tissues due to photobleaching process

Teck-Chee Chia; Zhiwei Huang; Wei Zheng; Cheong Hoong Diong; Francis Choen Seow

doi:10.1117/12.336838

19 January 1999 Changes in autofluorescence emission intensities of human colonic tissues due to photobleaching process

Teck-Chee Chia, Zhiwei Huang, Wei Zheng, Cheong Hoong Diong, Francis Choen Seow

Proceedings Volume 3568, Optical Biopsies and Microscopic Techniques III; (1999) https://doi.org/10.1117/12.336838
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

Changes in autofluorescence intensities at 550, 580, 680 and 720 nm as functions of incident intensity and exposure time were measured in normal mucosa and adenocarcinomatous of human colonic tissues when excited by the wavelengths of 457.9, 488, 514.5 and 632.8 nm, respectively. The obtained results show that the photobleaching process of the autofluorescence follows a double-exponential function. The slower decay rates of the photobleaching at 550 and 580 nm emissions could be found in normal tissues compared to those in tumor tissues, however, the faster decay times at 680 and 720 nm emissions were also found in normal tissues. It appears that the quantitative measurements of photobleaching processes may provide a method to evaluate the fractional contribution of the autofluorescence from different layers in the colon tissues. The evaluation of temporal behavior of photobleaching processes of autofluorescence emissions may also reveal the different accumulated concentrations of endogenous fluorophores between normal and tumor tissues.

Citation Download Citation

Teck-Chee Chia, Zhiwei Huang, Wei Zheng, Cheong Hoong Diong, and Francis Choen Seow "Changes in autofluorescence emission intensities of human colonic tissues due to photobleaching process", Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); https://doi.org/10.1117/12.336838

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available