Photoimmobilization of biomolecules with a scanning near-field optical microscope: functionalization and detection at the submicrometer scale

Claude Philipona; Patrick Lambelet; Luciana Vaccaro; Fabienne D. Marquis-Weible; Hans Sigrist

doi:10.1117/12.336937

15 January 1999 Photoimmobilization of biomolecules with a scanning near-field optical microscope: functionalization and detection at the submicrometer scale

Claude Philipona, Patrick Lambelet, Luciana Vaccaro, Fabienne D. Marquis-Weible, Hans Sigrist

Author Affiliations +

Proceedings Volume 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems; (1999) https://doi.org/10.1117/12.336937
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

In view of future generations of biosensors, immobilization of biomolecules with high spatial resolution onto selected materials or transducer surfaces is required. One way to attach the biomolecules is to induce covalent bonds with the substrate using a photochemical effect. High localization in the attachment is obtained when performing the photochemistry in the next field. In this paper, we present patterns of covalently bond proteins, written with near field UV activation. Observation is done in the near field as well, using near field fluorescence microscopy.

Citation Download Citation

Claude Philipona, Patrick Lambelet, Luciana Vaccaro, Fabienne D. Marquis-Weible, and Hans Sigrist "Photoimmobilization of biomolecules with a scanning near-field optical microscope: functionalization and detection at the submicrometer scale", Proc. SPIE 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems, (15 January 1999); https://doi.org/10.1117/12.336937

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