Optimization of 5-aminolaevulinic acid application and light excitation to ensure maximum contrast between cancerous and healthy tissue on the skin

Tracy McKechnie; Jacqueline Hewett; Wilson Sibbett; Miles J. Padgett; Colin Clark; James Ferguson

doi:10.1117/12.351513

6 July 1999 Optimization of 5-aminolaevulinic acid application and light excitation to ensure maximum contrast between cancerous and healthy tissue on the skin

Tracy McKechnie, Jacqueline Hewett, Wilson Sibbett, Miles J. Padgett, Colin Clark, James Ferguson

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Proceedings Volume 3592, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII; (1999) https://doi.org/10.1117/12.351513
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

This paper describes a low-cost system for the illumination and real-time imaging of skin lesions. The light source is a mercury arc lamp, filtered to preferentially excite the fluorescence of protoporphyrin IX (PpIX) formed within the skin after the topical application of 5-aminolaevulinic acid (ALA). A video camera with a reduced-frame-rate, i.e. integrating, enables low intensity fluorescence imaging. Appropriate filtering provides independent images of the illumination uniformity, PpIX fluorescence and autofluorescence. Subsequent image processing yields false color images of the tissue surface illustrating the extent of ALA application, tumor boarder, surrounding satellites and the possible demarcation of treatment zones.

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Tracy McKechnie, Jacqueline Hewett, Wilson Sibbett, Miles J. Padgett, Colin Clark, and James Ferguson "Optimization of 5-aminolaevulinic acid application and light excitation to ensure maximum contrast between cancerous and healthy tissue on the skin", Proc. SPIE 3592, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII, (6 July 1999); https://doi.org/10.1117/12.351513

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KEYWORDS

Luminescence

Skin

Optical filters

Photodynamic therapy

Tissue optics

Mercury

Tissues

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