Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy

Jean-Michel I. Maarek; Laura Marcu; Warren S. Grundfest M.D.; Michael C. Fishbein M.D.

doi:10.1117/12.351030

2 July 1999 Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy

Jean-Michel I. Maarek, Laura Marcu, Warren S. Grundfest M.D., Michael C. Fishbein M.D.

Proceedings Volume 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation; (1999) https://doi.org/10.1117/12.351030
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

In this study, we examine the possibility of differentiating between classes of atherosclerotic lesions based on time- resolved fluorescence spectroscopy and we compare the performance of classification schemes that use either the time-resolved spectra or only the intensity spectra. Transient fluorescence emissions induced by pulsed nitrogen laser excitation was measured on 87 excised samples of human aorta. The samples were classified histologically using the AHA classification Predictor variables derived from the time-resolved spectra included the spectral intensities at 360-510 nm and parameters of a biexponential fit of the fluorescence impulse response function. Stepwise discriminant analysis using these predict variables showed that a few predictor variables sufficed to correctly classify 89 percent of the samples. Excluding the time- dependent decay and using only the spectral intensities, the percentage of correctly classified cases was significantly lower: 51 percent. These results establish that time- resolved fluorescence spectroscopy markedly improved on the performance of steady-state fluorescence spectroscopy for fine classification of atherosclerotic lesions.

Citation Download Citation

Jean-Michel I. Maarek, Laura Marcu, Warren S. Grundfest M.D., and Michael C. Fishbein M.D. "Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); https://doi.org/10.1117/12.351030

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