Fluorescence lifetime measurements of NADH and tryptophan in intact ischemic, intact rabbit myocardium

Adrian Hamburger; Zygmunt Gryczynski; Joseph R. Lakowicz; Keith Sommers M.D.

doi:10.1117/12.351040

2 July 1999 Fluorescence lifetime measurements of NADH and tryptophan in intact ischemic, intact rabbit myocardium

Adrian Hamburger, Zygmunt Gryczynski, Joseph R. Lakowicz, Keith Sommers M.D.

Proceedings Volume 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation; (1999) https://doi.org/10.1117/12.351040
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Ischemia-reperfusion injury is the leading cause of early dysfunction following transplantation. Currently, there are no techniques available to accurately measure ischemic changes during organ storage. Therefore, the interest exists in developing non-invasive monitoring techniques. We used NADH and tryptophan as fluorescent markers, since both are intrinsic fluorophores and excellent indicators for levels of hypoxia and protein denaturation, respectively.

Citation Download Citation

Adrian Hamburger, Zygmunt Gryczynski, Joseph R. Lakowicz, and Keith Sommers M.D. "Fluorescence lifetime measurements of NADH and tryptophan in intact ischemic, intact rabbit myocardium", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); https://doi.org/10.1117/12.351040

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