Fluorescent imaging of cells and nucleic acids in bioelectronic chips

Jing Cheng; Lei Wu; Jonathan Diver; Paul N. Swanson; Adam Uribe; Michael Krihak; Jainamma Krotz; Kenneth A. Bloch; Bill Street; Trevor Young; Tony Llevares; Tim LeClair; Donald E. Ackley; Michael J. Heller; James P. O'Connell; Tina S. Nova

doi:10.1117/12.351041

2 July 1999 Fluorescent imaging of cells and nucleic acids in bioelectronic chips

Jing Cheng, Lei Wu, Jonathan Diver, Paul N. Swanson, Adam Uribe, Michael Krihak, Jainamma Krotz, Kenneth A. Bloch, Bill Street, Trevor Young, Tony Llevares, Tim LeClair, Donald E. Ackley, Michael J. Heller, James P. O'Connell, Tina S. Nova

Author Affiliations +

Proceedings Volume 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation; (1999) https://doi.org/10.1117/12.351041
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We have performed separation of bacterial and cultured cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also related DNA and RNA from the isolated cells electrically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts toward the construction of a 'laboratory-on- a-chip' system will be presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

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Jing Cheng, Lei Wu, Jonathan Diver, Paul N. Swanson, Adam Uribe, Michael Krihak, Jainamma Krotz, Kenneth A. Bloch, Bill Street, Trevor Young, Tony Llevares, Tim LeClair, Donald E. Ackley, Michael J. Heller, James P. O'Connell, and Tina S. Nova "Fluorescent imaging of cells and nucleic acids in bioelectronic chips", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); https://doi.org/10.1117/12.351041

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