Long-lifetime and long-wavelength osmium (II) metal complexes containing polypyridine ligands: excellent red fluorescent dyes for biophysics and for sensors

Zakir Murtaza; Joseph R. Lakowicz

doi:10.1117/12.347549

3 May 1999 Long-lifetime and long-wavelength osmium (II) metal complexes containing polypyridine ligands: excellent red fluorescent dyes for biophysics and for sensors

Zakir Murtaza, Joseph R. Lakowicz

Author Affiliations +

Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347549
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Several luminescent complexes of osmium (II) containing polypyridine ligands have been prepared. The syntheses, photophysical and fluorescence polarization properties of [Os(phen)₂(aphen)]²⁺, [Os(tpy)(mcbpy)(py)]²⁺, [Os(ttpy)₂]²⁺, [Os(tpy)(triphos)]²⁺ and [Os(tppz)₂]²⁺ are reported. Where phen is 1,10-phenanthroline, aphen is 5-amino-1,10-phenanthroline, tpy is 2,2':6,2'-terpyridine, mcbpy is 4-methyl-2,2'- bipyridine-4'-carboxylic, py is pyridine, triphos is bis(2-diphenylphosphinoethyl)-phenyl phosphine, ttpy is 4- tolyl,2,2':6,2'-terpyridine, and tppz is 2,3,5,6- tetrakis(2-pyridyl)pyrazine. The complexes absorb light at above 550 nm, emit above 700 nm, and have emission lifetimes longer than 50 ns in water. The emission of all the complexes is polarized, so they can have applications as red light excitable dyes for biophysical studies of macromolecules and for polarization immunoassays. The complexes can also be used for lifetime-based oxygen sensing using low-cost phase fluorometry and a LED light source.

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Zakir Murtaza and Joseph R. Lakowicz "Long-lifetime and long-wavelength osmium (II) metal complexes containing polypyridine ligands: excellent red fluorescent dyes for biophysics and for sensors", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); https://doi.org/10.1117/12.347549

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