Highly efficient optical detection of surface-generated fluorescence

Joerg Enderlein; Thomas Ruckstuhl; Stefan Seeger

doi:10.1117/12.346729

21 April 1999 Highly efficient optical detection of surface-generated fluorescence

Joerg Enderlein, Thomas Ruckstuhl, Stefan Seeger

Proceedings Volume 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II; (1999) https://doi.org/10.1117/12.346729
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We describe a new optical system for the detection of laser- induced fluorescence of surface bound molecules. The system consists of two optical elements: a paraboloid glass segment, and a spherical long focal lens. The function of the paraboloid glass segment is twofold: First, due to jump in refractive index at the water-glass interface, surface bound molecules emit the major part of their fluorescence into the paraboloid. Second, the parabolic shape of the segment services as a collecting mirror. We demonstrate that with this simple system an extremely high light collection efficiency can be achieved, without using sophisticated and expensive objectives with high numerical aperture. Ray- tracing calculations show that the system is able of confocal imaging, which is very important for efficient suppression of scattered excitation light. Experimental result showing the exquisite performance and sensitivity are presented.

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Joerg Enderlein, Thomas Ruckstuhl, and Stefan Seeger "Highly efficient optical detection of surface-generated fluorescence", Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); https://doi.org/10.1117/12.346729

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KEYWORDS

Luminescence

Molecules

Glasses

Fluorescence spectroscopy

Interfaces

Refractive index

Spherical lenses

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