Spatial point spread function with depth in two-photon microscopy

Andrew K. Dunn; Vincent P. Wallace; Mariah L. Coleno; Peter T. C. So; Bruce J. Tromberg

doi:10.1117/12.347559

6 May 1999 Spatial point spread function with depth in two-photon microscopy

Andrew K. Dunn, Vincent P. Wallace, Mariah L. Coleno, Peter T. C. So, Bruce J. Tromberg

Author Affiliations +

Proceedings Volume 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI; (1999) https://doi.org/10.1117/12.347559
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

The spatial point spread function is studied for two-photon fluorescence imaging in turbid media. Using a Monte Carlo based model the effects of tissue optical properties on the point spread function, fluorescence generation, and fluorescence collection are studied. The simulated results are compared to measured point spread functions at different depths within a scattering sample. Results indicate that the limiting factor in two-photon imaging is a loss of signal caused by scattering rather than a loss of resolution.

Citation Download Citation

Andrew K. Dunn, Vincent P. Wallace, Mariah L. Coleno, Peter T. C. So, and Bruce J. Tromberg "Spatial point spread function with depth in two-photon microscopy", Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); https://doi.org/10.1117/12.347559

Access the abstract

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY