Sensitivity enhancement of transducers for total internal reflection fluorescence

Albrecht Klotz; C. Barzen; Andreas Brecht; Richard D. Harris; G. R. Quigley; James S. Wilkinson; Guenter Gauglitz

doi:10.1117/12.343754

26 March 1999 Sensitivity enhancement of transducers for total internal reflection fluorescence

Albrecht Klotz, C. Barzen, Andreas Brecht, Richard D. Harris, G. R. Quigley, James S. Wilkinson, Guenter Gauglitz

Proceedings Volume 3620, Integrated Optics Devices III; (1999) https://doi.org/10.1117/12.343754
Event: Optoelectronics '99 - Integrated Optoelectronic Devices, 1999, San Jose, CA, United States

Abstract

We have developed, modeled and optimized optical transducers for total internal reflection fluorescence (TIRF). The transducers are part of a compact and rugged immuno-analytical instrument designed for simultaneous detection of up to six analytes in aquatic samples (e.g. atrazine and 2,4-D). Binding inhibition assays, using Cy5.5 labeled antibodies to detect the target analytes, have been carried out. Calibration curves with mid-points of tests below 1 (mu) g/1 and detection limits below 0.1 (mu) g/1 have been achieved. As transducer either ion exchanged integrated optical channel waveguides or planar multimode slab waveguides have been employed. The transducer performance was significantly enhanced by incorporating thin high index films at the waveguide surface and by applying high refractive index solutions in the superstrate. Peak signal enhancement factors of more than ten have been observed and an increase in signal to noise ratio by a factor of more than four have been achieved. Strong polarization dependent effects on the enhancement by high index films have been found both theoretically and experimentally.

Citation Download Citation

Albrecht Klotz, C. Barzen, Andreas Brecht, Richard D. Harris, G. R. Quigley, James S. Wilkinson, and Guenter Gauglitz "Sensitivity enhancement of transducers for total internal reflection fluorescence", Proc. SPIE 3620, Integrated Optics Devices III, (26 March 1999); https://doi.org/10.1117/12.343754

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available