Tissuelike phantoms: fluorescence under 405-nm excitation

Ludymila P. Danilova; Svetlana P. Chernova; Alexander B. Pravdin

doi:10.1117/12.341447

4 March 1999 Tissuelike phantoms: fluorescence under 405-nm excitation

Ludymila P. Danilova, Svetlana P. Chernova, Alexander B. Pravdin

Proceedings Volume 3726, Saratov Fall Meeting '98: Light Scattering Technologies for Mechanics, Biomedicine, and Material Science; (1999) https://doi.org/10.1117/12.341447
Event: Saratov Fall Meeting '98: Light Scattering Technologies for Mechanics, Biomedicine, and Material Science, 1998, Saratov, Russian Federation

Abstract

In this work, as a part of complex work aimed at the development of tissue-like phantoms of biotissue and evaluation of their fluorescence properties, we obtained fluorescence spectra (over 480 - 670 nm interval) of the phantoms and their components under 405 +/- 20 nm excitation. Fluorescence escape from the layer modeling collagenous stroma with capillary network, as dependent on blood content in it, was studied. It was shown that collagen added to upper layers of phantom causes an uniform increase of intensity over whole spectral range under study, whereas the addition of NADH to `epithelium' results in the increase in 480 - 580 nm interval only.

Citation Download Citation

Ludymila P. Danilova, Svetlana P. Chernova, and Alexander B. Pravdin "Tissuelike phantoms: fluorescence under 405-nm excitation", Proc. SPIE 3726, Saratov Fall Meeting '98: Light Scattering Technologies for Mechanics, Biomedicine, and Material Science, (4 March 1999); https://doi.org/10.1117/12.341447

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