Real-time confocal two-photon fluorescence microscope using a rotating microlens array

Katsumasa Fujita; Tomoyuki Kaneko; Osamu Nakamura; Masahito Oyamada; Tetsuro Takamatsu; Satoshi Kawata

doi:10.1117/12.347844

7 May 1999 Real-time confocal two-photon fluorescence microscope using a rotating microlens array

Katsumasa Fujita, Tomoyuki Kaneko, Osamu Nakamura, Masahito Oyamada, Tetsuro Takamatsu, Satoshi Kawata

Proceedings Volume 3740, Optical Engineering for Sensing and Nanotechnology (ICOSN '99); (1999) https://doi.org/10.1117/12.347844
Event: Optical Engineering for Sensing and Nanotechnology (ICOSN '99), 1999, Yokohama, Japan

Abstract

The use of a confocal pinhole-array in a real-time two-photon fluorescence microscope with a microlens array is proposed to increase the three-dimensional resolution and the depth- penetration property. Enhancement of the lateral and axial resolution in the proposed microscope is observed in our experiments. Comparisons of images taken with and without the pinhole-array to verify the sharp depth-imaging performance are shown.

Citation Download Citation

Katsumasa Fujita, Tomoyuki Kaneko, Osamu Nakamura, Masahito Oyamada, Tetsuro Takamatsu, and Satoshi Kawata "Real-time confocal two-photon fluorescence microscope using a rotating microlens array", Proc. SPIE 3740, Optical Engineering for Sensing and Nanotechnology (ICOSN '99), (7 May 1999); https://doi.org/10.1117/12.347844

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available