Integrated optical toxin sensor

Dan Kelly; Xuedong Song; Daniel Keith Frayer; Sergio Brito Mendes; Nasser Peyghambarian; Basil I. Swanson; Karen M. Grace

doi:10.1117/12.372899

15 December 1999 Integrated optical toxin sensor

Dan Kelly, Xuedong Song, Daniel Keith Frayer, Sergio Brito Mendes, Nasser Peyghambarian, Basil I. Swanson, Karen M. Grace

Author Affiliations +

Proceedings Volume 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection; (1999) https://doi.org/10.1117/12.372899
Event: Photonics East '99, 1999, Boston, MA, United States

Abstract

We have developed a method for simple and highly sensitive detection of multivalent proteins using an optical waveguide sensor. The optical biosensor is based on optically tagged glycolipid receptors imbedded within a fluid phospholipid bilayer membrane formed on the surface of a planar optical waveguide. The binding of multivalent toxin initiates a fluorescence resonance energy transfer resulting in a distinctive spectral signature that is monitored by measuring emitted luminescence above the waveguide surface. The sensor methodology is highly sensitive and specific, and requires no additional reagents or washing steps. Demonstration of the utility of protein-receptor recognition using planar optical waveguides is shown here by the detection of cholera toxin.

Citation Download Citation

Dan Kelly, Xuedong Song, Daniel Keith Frayer, Sergio Brito Mendes, Nasser Peyghambarian, Basil I. Swanson, and Karen M. Grace "Integrated optical toxin sensor", Proc. SPIE 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection, (15 December 1999); https://doi.org/10.1117/12.372899

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available