Immobilization of a calcein derivative onto optical fibers for calcium ion sensing

William D. Sloan; Mahesh Uttamlal

doi:10.1117/12.372991

9 December 1999 Immobilization of a calcein derivative onto optical fibers for calcium ion sensing

William D. Sloan, Mahesh Uttamlal

Proceedings Volume 3860, Fiber Optic Sensor Technology and Applications; (1999) https://doi.org/10.1117/12.372991
Event: Photonics East '99, 1999, Boston, MA, United States

Abstract

This paper describes a new fiber-optic calcium ion sensor using the calcein derivative, calcein acrylamide. Calcein acrylamide (CA) is a fluorescent indicator with a sensitivity for calcium ions at pH greater than 10. Ca²⁺ binds with CA with a binding ratio of 2:1 ratio therefore the concentration range for Ca²⁺ sensing depends on the amount of indicator present. Typically, calcium can be detected in the range 0 to 20 micrometers ol 1^-1. The indicator can be covalently immobilized within a polymer matrix which itself is covalently immobilized onto a chemically modified optical fiber using photo-initiated polymerization. Three Ca²⁺ sensitive polymers are presented; CA in (1) poly-hydroxyethyl methacrylate (poly- HEMA), (2) poly-acrylamide and (3) poly-N-vinyl-pyrrolidinone (n-VP). The sensitivity of the immobilized CA are similar to that of CA in free solution.

Citation Download Citation

William D. Sloan and Mahesh Uttamlal "Immobilization of a calcein derivative onto optical fibers for calcium ion sensing", Proc. SPIE 3860, Fiber Optic Sensor Technology and Applications, (9 December 1999); https://doi.org/10.1117/12.372991

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