Microfluidic approaches to immunoassays

Anson Hatch; Bernhard H. Weigl; Diane Zebert; Paul Yager

doi:10.1117/12.359334

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19 August 1999 Microfluidic approaches to immunoassays

Anson Hatch, Bernhard H. Weigl, Diane Zebert, Paul Yager

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Proceedings Volume 3877, Microfluidic Devices and Systems II; (1999) https://doi.org/10.1117/12.359334
Event: Symposium on Micromachining and Microfabrication, 1999, Santa Clara, CA, United States

Abstract

An immunoassay format is presented that takes advantage of the microfluidic properties of the H-Filter^TM for measuring sample analyte concentration. The method relies on the diffusion of analyte particles into a region containing beads coated with specific antibody. Competitive binding of labeled analyte and sample analyte with a limited number of binding sites allows measurement of the concentration of sample analyte based on the fraction of labeled analyte bound. The fraction of labeled analyte bound can be determined with a microcytometer by measuring the bead fluorescence intensity on the microcytometer portion of an integrated microfluidic chip. It is not necessary to separate the beads from the mixture because the bead intensity can be determined above the background of unbound labeled antigens. Other advantages include the ability to eliminate large interfering particles from samples, continuous sample monitoring, and the ability to concentrate the beads. Microfluidic immunoassay formats also consume smaller volumes of costly reagents and sample.

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Anson Hatch, Bernhard H. Weigl, Diane Zebert, and Paul Yager "Microfluidic approaches to immunoassays", Proc. SPIE 3877, Microfluidic Devices and Systems II, (19 August 1999); https://doi.org/10.1117/12.359334

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