Colorectal adenocarcinoma diagnosis by FT-IR microspectrometry

Peter Lasch; Juergen Schmitt; Dieter Naumann

doi:10.1117/12.384948

8 May 2000 Colorectal adenocarcinoma diagnosis by FT-IR microspectrometry

Peter Lasch, Juergen Schmitt, Dieter Naumann

Proceedings Volume 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques; (2000) https://doi.org/10.1117/12.384948
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The feasibility of characterizing human colorectal adenocarcinoma by IR microspectrometry is described. Carcinoma thin sections were analyzed by spatially resolved mid-IR FT microspectrometry, and for comparative purposes by conventional histological staining techniques. More than 2300 high quality FTIR reference spectra of 27 patient samples form 11 defined morphological structures such as crypts, tunica muscularis, submucosa and adenocarcinoma were recorded. The analysis of the spectral data included four steps: an initial test for spectral quality, data preprocessing, data reduction and classification of the tissue spectra by pattern recognition techniques. The overall classification accuracy attainted by an optimized ANN of about 95 percent highlights the great potential of FTIR microspectrometry as a diagnostic tool for the determination of a variety of tissue structures. Further improvements are necessary to make the new method applicable to routine and experimental clinical analysis in the future.

Citation Download Citation

Peter Lasch, Juergen Schmitt, and Dieter Naumann "Colorectal adenocarcinoma diagnosis by FT-IR microspectrometry", Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); https://doi.org/10.1117/12.384948

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