Adaptive compensation of aberrations in ultrafast 3D microscopy using a deformable mirror

Leah R. Sherman; O. Albert; Christoph F. Schmidt; Gleb V. Vdovin; Gerard A. Mourou; Theodore B. Norris

doi:10.1117/12.384186

2 May 2000 Adaptive compensation of aberrations in ultrafast 3D microscopy using a deformable mirror

Leah R. Sherman, O. Albert, Christoph F. Schmidt, Gleb V. Vdovin, Gerard A. Mourou, Theodore B. Norris

Proceedings Volume 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII; (2000) https://doi.org/10.1117/12.384186
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

3D imaging using a multiphoton scanning confocal microscope is ultimately limited by aberrations of the system. We describe a system to adaptively compensate the aberrations with a deformable mirror. We have increased the transverse scanning range of the microscope by three with compensation of off-axis aberrations.We have also significantly increased the longitudinal scanning depth with compensation of spherical aberrations from the penetration into the sample. Our correction is based on a genetic algorithm that uses second harmonic or two-photon fluorescence signal excited by femtosecond pulses from the sample as the enhancement parameter. This allows us to globally optimize the wavefront without a wavefront measurement. To improve the speed of the optimization we use Zernike polynomials as the basis for correction. Corrections can be stored in a database for look-up with future samples.

Citation Download Citation

Leah R. Sherman, O. Albert, Christoph F. Schmidt, Gleb V. Vdovin, Gerard A. Mourou, and Theodore B. Norris "Adaptive compensation of aberrations in ultrafast 3D microscopy using a deformable mirror", Proc. SPIE 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII, (2 May 2000); https://doi.org/10.1117/12.384186

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